Translational Pathology Spatial Proteomics CLIA-certified Core

Multiparametric In Situ Imaging (MISI) Laboratory

MISI couples diagnostic tissue pathology expertise with high-dimensional spatial imaging to characterize human tissues at single-cell resolution. Embedded in the clinical environment, MISI accelerates discovery while maintaining pathologist oversight and clinical relevance.

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What MISI enables

Quantitative, spatially resolved protein profiling in intact tissue architecture
High-plex immunofluorescence with validated, automated workflows
Datasets ready for computational pathology and AI-enabled analysis

2019

Established

Vectra markers

36x

COMET markers

Focus Platforms Services Resources Faculty Contact

Spatial biology embedded in clinical pathology

Scientific focus

Microenvironments in context

Map immune, stromal, and tumor compartments while preserving spatial relationships and tissue architecture.

Quantitative pathology at scale

Standardized, automated staining and acquisition pipelines support reproducibility across slides, cohorts, and studies.

Translation-ready outputs

Produce analysis-ready outputs for downstream computational pathology and AI workflows within the broader CTP ecosystem.

Positioning statement

By embedding advanced spatial imaging directly within the clinical pathology environment, MISI transforms diagnostic tissue into a high-resolution discovery platform-bridging patient care and precision research.

High-plex spatial imaging with automated workflows

Platforms & technologies

Vectra Polaris(R)

Automated quantitative pathology imaging system

Up to 7 markers

Simultaneous interrogation of up to 7 protein biomarkers
Fully automated workflow
High-throughput whole-slide and TMA imaging
40x resolution for single-cell quantification

Lunaphore COMET(TM)

High-plex immunofluorescence staining + acquisition

Up to 36 markers

High-plex immunofluorescence detection (up to 36 markers)
No conjugation required for primary antibodies
Curated primary antibody panels provided by MISI core (custom panels available)
Completely automated workflow for staining and acquisition
Designed for FFPE tissue
Processes 1-4 slides per run
High-sensitivity camera; acquisition window 12.5 x 12.5 mm

From study design to analysis-ready deliverables

Services

Multiplex immunofluorescence staining

Whole-slide and region-based imaging

Quantitative image analysis

Study design consultation

Integration with computational pathology pipelines

CLIA-certified environment and standardized workflows

Fees

Fee schedules and service details are available upon request.

How to engage MISI

1
Contact the CTP intake to discuss goals, tissue type, and marker requirements
2
Align on workflow, run plan, and deliverables
3
Submit samples with checklist and required metadata
4
Receive images + quantified outputs for downstream analysis

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Templates and reference documents

Resources

List of markers available PDF

Current validated marker availability for high-plex workflows

Sample submission checklist PDF

Required metadata and pre-submission QC items

Publications

Add MISI-related publications here (e.g., curated list or links to PubMed). If you want a consistent site-wide pattern, consider reusing the same publications component you use elsewhere in CTP.

Scientific and clinical oversight